胎牛视网膜干细胞向神经细胞诱导研究

doi:10.11843/j.issn.0366-6964.2014.04.008

Abstract

Abstract:

This study aimed to research the biological characteristics of retinal stem cells (RSCs) from bovine fetus.RSCs were isolated using physical method，and the surface markers of RSCs，Nestin，Chx-10 and Pax-6，were detected by immunofluorescence.The multi-lineage differentiation potential of RSCs was investigated and induced to differentiate into astrocytes，neurons and oligodendrocytes.The results showed that the fetal bovine RSCs not only expressed the special markers of stem cells but also induced to astrocytes，neurons and oligodendrocytes successfully.To sum up the RSCs isolated from fetal bovine have a high proliferation and multi-directional differentiation potential in vitro.Retinal stem cells for the future research provide the corresponding theoretical foundation.

CLC Number:

S823
Q42

SUN Bo，ZHANG Wen-xiu，WU Fang-chun，MA Yue-hui，SHA Wei，GUAN Wei-jun. Directed Neurons Differentiation of Bovine Retinal Stem Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 559-565.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.04.008

https://www.xmsyxb.com/EN/Y2014/V45/I4/559

References

［1］TAKAHASHI K，YAMANAKA S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors［J］.Cell， 2006,126:663-676.
［2］MURAI K K,PASQUALE E B.Eph receptors and ephrins in neuron-astrocyte communication at synapses［J］.Glia，2011，59(11)：1567-1578
［3］郑月茂，兰志刚，赵晓娥，等.猪胎儿神经干细胞的分离培养和分化［J］.畜牧兽医学报，2009，40(11)：1686-1689.
［4］LAMBA D A，KARL M O，WARE C B，et al.Efficient generation of retinal progenitor cells from human embryonic stem cells［J］. Proc Natl Acad Sci U S A ,2006,103(34):12769-12774.
［5］TUCKER B A，PARK I H，QI S D，et al.Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in degenerative mice［J］.PLoS ONE，2011,6(4):e18992.
［6］石芊,彭秀军.新生小鼠视网膜光感受器前体细胞的体外培养及鉴定［J］.解放军医学杂志,2009,34(11):1340-1342.
［7］GONG X，HOU L,BAI C，et al.Isolation and biological characteristic of chicken adipose-derived progenitor cells ［J］.DNA Cell Biol ,2011,30:453-470
［8］LI L，BAI X GONG X，et al.Differentiation potential of bone marrow mesenchymal stem cells in duck［J］.J Genet Genom,2009,36:133-140
［9］肖迎,唐仕波,黄冰,等.胚胎干细胞直接向神经细胞诱导分化的试验研究［J］.中国病理生理杂志,2006,22(11):2081-2085.
［10］XU S，SUNDERLAND M E,COLES B L，et a1．The proliferation and expansion of retinal stem cells require functional Pax6［J］．Dev Biol,2007,304：713-721．
［11］VOSSMERBAEUMER U，KUEHL S，KERN S,et al.Induction of retinal pigment epithelium properties in ciliary margin progenitor cells［J］.Clin Exp Ophthalmol，2008,36(4):358-366.
［12］ALI R R，SOWDEN J C.Regenerative medicine：DIY eye［J］.Nature,2011，472(7341):42-43.
［13］PHILLIPS M J，OTTESON D C.Differential expression of neuronal genes in Müller glia in two-and three-dimensional cultures［J］.Invest Ophthalmol Vis Sci,2011,52(3):1439-1449.
［14］ANGÉNIEUX B，SCHORDERET D F，ARSENIJEVIC Y.Epidermal growth factor is a neuronal differentiation factor for retinal stem cells in vitro［J］.Stem Cells,2006,24(3):696-706.
［15］JIAN Q，XU H，XIE H，et al.Activation of retinal stem cells in the proliferating marginal region of RCS rats during development of retinitis pigmentosa［J］.Neurosci Lett，2009,465(1):41-44.
［16］计菁,罗敏,冯霞.大鼠胚胎视网膜中神经干细胞的体外培养与鉴定［J］.眼科新进展,2008,28(6):429-431.
［17］DESMAISON A，VIGOUROUX A，RIEUBLAND C,et al.Mutations in the LHX2 gene are not a frequent cause of micro/anophthalmia［J］.Mol Vis,2010,16:2847-2849.
［18］REICHMAN S，KALATHUR R K，LAMBARD S,et al.The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina［J］.Hum Mol Genet, 2010,19(2):250-261.
［19］CHEN H L，YUH C H，WU K K.Nestin is essential for zebrafish brain and eye development through control of progenitor cell apoptosis［J］.PLoS ONE，2010,5(2):e9318.

[1]	CAO Jianhua, YANG Baigao, ZHANG Peipei, FENG Xiaoyi, ZHANG Hang, YU Zhou, NIU Yifan, HAO Haisheng, DU Weihua, ZHU Huabin, YANG Ling, ZHAO Xueming. Mechanisms of Negative Energy Balance Affects Follicular Development in Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 22-30.
[2]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[3]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[4]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[5]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[6]	ZHANG Wentao, LIU Chenyang, ZHU Binglin, LIU Li, TIAN Yuan, YAO Yuhang, CHENG Gong. Study on the Effects and Mechanisms of Snail1 on Proliferation and Differentiation of Bovine Adipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5008-5019.
[7]	NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.
[8]	WANG Wenxiang, DU Lili, HU Junwei, ZHANG Yanxiang, MA Minghao, DUAN Rui, QIAN Cong, WANG Xinyue, LI Sanlu, ZHANG Changqing, ZHANG Lupei, GAO Xue, XU Lingyang, LI Junya, GAO Huijiang. Mining Candidate Genes Related to Meat Quality Traits of Longissimus Lumborum in Pingliang Red Steer Based on Transcriptome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4589-4604.
[9]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Transcriptome Analysis of the Cerebral Cortex in Newborn and Adult Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4605-4614.
[10]	DONG Zhihao, SHI Yuxin, GUO Guanhua, YUAN Kaimin, XIU Haoyu, WANG Chao, BAI Junyan, WANG Dong. Comparative Analysis of Salivary Compounds in Different Estrous Stages of Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4636-4652.
[11]	SUN Dongxiao, ZHANG Shengli, ZHANG Qin, LI Jiao, ZHANG Guixiang, LIU Chousheng, ZHENG Weijie. Application Progress on Genomic Selection Technology for Dairy Cattle in China [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4028-4039.
[12]	FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Progress in Mechanism of Reduced Fertility in Dairy Cows due to Negative Energy Balance [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4050-4060.
[13]	MA Haoran, ZHANG Lupei, JIN Shengyun, BAO Jinshan, LI Hongyan, GAO Huijiang, XU Lingyang, WANG Zezhao, LI Junya. Assessment of the Genomic Relationships for Chinese Indigenous Beef Cattle Using High-density SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4174-4185.
[14]	GUO Yixin, WANG Zhisheng, HU Rui, WANG Junmei, WANG Sen, SHI Liyuan, ZHANG Xiaohong, ZOU Huawei, ZUO Jiaxue, PENG Quanhui, XUE Bai, WANG Lizhi. Effect of Leucine on Browning of Subcutaneous Adipocytes in Yellow Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3286-3298.
[15]	CHU Chu, ZHANG Jingjing, DING Lei, FAN Yikai, BAO Xiangnan, XIANG Shixin, LIU Rui, LUO Xuelu, REN Xiaoli, LI Chunfang, LIU Wenju, WANG Liang, LIU Li, LI Yongqing, JIANG Han, LI Weiqi, SUN Wei, LI Xihe, WEN Wan, ZHOU Jiamin, ZHANG Shujun. Establishment and Application of Prediction Model of Three Amino Acids in Milk Based on Mid-infrared Spectroscopy [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3299-3312.

Directed Neurons Differentiation of Bovine Retinal Stem Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments